The Effect of Hydro Ethanolic Seeds Extract of Neem(Azadirachta indica) and Bakain (Melia azedarach) to the Number of Trypanosoma evansi Steel in Liver of Mice

Inroduction & Objective:

Trypanosoma evansi induced trypanosomiasis causes infection in domestic mammals as well as humans. Treatment of trypanosomiasis has limitations, including resistance to chemical drugs. The aim of this study was to identify active natural products and compare the effects of hydroalcoholic extracts of neem seeds and bitter olives against the resistant agent of this disease in the liver organ of mice infected with Trypanosoma evansi.

After collecting and identifying neem and Bakain seeds plants, hydroalcoholic extract was prepared from their seeds by Perkoleh method and after concentrating 100-500 mg / kg of rat weight peritoneally to male rats infected with 3×107 Trypanosoma evansi were injected for 3 days. Routine method was used to determine the number of noises. Serum levels of ALT, AST, ALP were measured. Tissue pathology tests of. Histopathological liver tests were performed after hematoxylin and eosin staining. Statistical evaluation was performed using ANOVA analysis of variance and Prism software and the error limit of  p <0.05 was accepted as a significant difference.

Neem and Bakain seeds extracts both reduced the number of parasites and compared to the two extracts, the reducing effect of Bakain was greater than that of Neem. Congestion changes, fat degeneration, vacuolar degeneration, Necrosis and cell infiltration in the liver nephritis was higher of the control groups than the treated groups.

The use of neem and Bakain seeds hydroalcoholic extracts of in a certain amount of herbal medicine is effective in trypanosomosis (soda).