The effect of adding hypotaurine antioxidant on reduction of lipid peroxidation and improving motility of Rooster sperm during freezing and thawing

The effect of adding hypotaurine antioxidant on reduction of lipid peroxidation and improving motility of Rooster sperm during freezing and thawingThe aim of the present study was to investigate the antioxidant effect of hypotaurine at levels of 0.25, 0.5, 0.75 and 1 mM in lecithin-based Lake extender during the freezing and thawing process on motility, survival, plasma membrane integrity, the amount of MDA in the semen and sperm morphology parameters. For this purpose, semen samples were collected from 15 roosters by by abdominal massage method twice a week. After diluting and addation of different levels of antioxidants, the samples were frozen on liquid nitrogen vapor. After thawing, the samples were evaluated for sperm performance parameters. Results indicated an increase in sperm velocity parameters, total motility, progressive motility, viability, membrane integrity by adding 0.75 mM/L hypotaurine antioxidant compared with the other groups (P<0.05). Also, the level of 0.75 mM was significantly reduced (P <0.05) in the amount of MDA in the semen and the highest level was obtained in terms of these parameters at this level. The results showed that adding the optimal level of hypotaurine could play a protective role against lipid peroxidation and improve the appearance of rooster sperm during freezing and thawing and reduce the damage caused by oxidative stress.