Allopatic effect of Artemisia aucheri essential oil on seed germination characteristics of Medicago sativa var.blak
Allergic action of a plant on adjacent plants can have negative effects on their growth and development. Also, alien harmful compounds play an important role in biodiversity and the ability of ecosystems to produce.Allelopathic compounds play a major role in biodiversity and the ability to produce ecosystems. Accordingly, in this research, the effect of medicinal and essential oil of Artemisia aucheri on early germination of Medicago sativa var.blak was investigated. Clevenger was used to prepare essential oil. Factorial experiment was conducted in a completely randomized design with two factors of essential oil concentration (level 0 (control), 100 ppm, 200 ppm, 300 ppm, 400 ppm, 500 ppm). 4 replicates of 50 seeds of the Medicago sativa var.blak were placed on two layers of Watman 42 paper inside the petri dishes with a diameter of 10 cm. Samples were placed under controlled germinator conditions with a temperature of 15-25 ° C, a relative humidity of 95%, and a light period of 8 hours of light and 16 hours of darkness. Seed counting continued until the 14th day to determine the germination characteristics and early growth of alfalfa seeds. The results showed that the highest germination percentage, germination rate, straw index, mean shoot length and mean root length were related to control treatment. In this treatment, the shortest time was 1/56 days. Germination percentage was 94% in control treatment. Black alfalfa seeds continued to sprout up to a concentration of 300 ppm. The germination rate, germination rate and indigenous index, mean shoot growth and root growth were minimized at this concentration. No germination percentage was obtained at a concentration of 500 ppm. Germination stopped completely at 400 and 500 ppm. Considering that Dashti Artemisia species has covered a wide range of rangelands in Iran, it is recommended to identify the hazardous compounds and determine their amount in these species in relation to proper rangeland management.
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