Investigation on the effects of growth regulators on morphological characteristics and antioxidant compounds of Lilium ledebourii Bioss under in vitro conditions

Lilium ledebourii is a native ornamental plant in Iran that has medicinal properties beside ornamental aspects. In the present study, the effects of different concentrations of BA on morphologic traits and secondary metabolite production in L. ledebeourii were evaluated. For this purpose, MS medium Supplemented with 1 mg/l NAA and 1, 2, 3 and 4 mg/l BA has been used. Results showed that maximum number and length of roots and Highest indexes of leaves (number, area and length), plantlet height, number and diameter of bulblets, number of scales, chlorophyll, Anthocyanin, Total phenol and flavonoid contents were obtained in control medium and medium Contained 1 mg/ l BA, respectively. With increasing concentrations of BA in the medium, previously described indexes were enhanced. Also, direct regression observed between chlorophyll content and Anthocyanin, Flavonoids and total phenols. This indicated a key role of photosynthesis in secondary metabolite production under in vitro condition. Generally, It Seems necessary that determined optimum Cytokinin concentration and Cytokinin/Auxin ratio in the Culture medium.