Using cheap by-products of food industry for cultivation of Paenibacillus polymyxa N179

In current research Paenibacillus polymyxa strain N179 was selected based on its previous performance in greenhouse and field condition, and cheap by-products of food industry was used to introduce a best medium for its high quality and low cost cultivation. The growth rate of this bacterium in various media was significantly different. In addition, antagonistic activity of cells from various media either in-vitro or in greenhouse condition was significantly different. The cells from M4 (10 g date pomace, 4 g sesame press cake) and M10 (4 g sesame press cake, 5 g starch) have shown highest inhibition against Rhizoctonia solani fungus. The resistance of cells from media against formulation process and viability during preservation period was assessed and it was found that cells from LB, M9, M7, and M2 media were the most resistant ones during preservation period. Moreover, efficacy of prepared formulations was assessed after three months in greenhouse condition and it was found that formulations made from M5 and LB were the most effective ones in controlling the disease. However, considering all of the results, M9 was shown the best performance.