Comparison of spring and autumn Artemisia aucheri extracts in in inhibition of HSV- 1 virus replication

The use of plants as medicine has always been considered. In the present study, the effect of aqueous extract collected in spring and autumn of Artemisia aucheri on the survival of HSV- 1 virus-infected cells was investigated.

In this study, the effect of aqueous extract of Artemisia aucheri in spring and autumn on the survival of Vero cell line after HSV- 1 infection at different times and concentrations was compared using TCID50 and MTT methods. The effect of aqueous extract of Artemisia aucheri at concentrations of 25, 50, 75, and 100 mg / ml on Vero cell line infected with HSV- 1 virus was performed for 24 hours in DMEM medium containing 2% (FBS) and then a dose of 50% Tissue Culture Infectious Dose(TCID50) was determined. Cell viability was also assessed by MTT assay at 2-, 0, 4, 8, and 24 hours.

The dose of 50% TCID50 with different dilutions of spring and autumn aqueous extracts of Artemisia aucheri was determined on Vero cells infected with (MOI = 0.1) HSV- 1 virus by inverted microscope and compared by MTT method. Concentrations of 50, 75, µg/ml and 100 µg/ml of spring extract and concentrations of 50 and 75 µ g / ml of autumn extract significantly reduced viral infection. Among them, spring extract with a concentration of 75 µ g / ml showed the highest viral inhibition and cell survival. Also, a significant difference was observed between spring and autumn aqueous extracts of Artemisia aucheri on cell survival (r = 0.97, P =0.0002).

The results indicated that the spring aqueous extract of Artemisia aucheri has a significant anti-HSV- 1 antiviral effect compared to the autumn aqueous extract of Artemisia aucheri (A = 0.0001) (P = 0.0001).