The Effect of Intense Endurance Training and Intense Periodic Oxygenase-1 and Tumor Necrosis Factor-α in the Heart Tissue of Male Wistar Rats

Exercise is one of the effective factors in causing oxidative stress. Increased oxidative stress can be effective in the development of injuries and cardiovascular disease and stimulate the production of cytokines from many cells, but on the other hand to counteract these pressures, the body responds to oxidative stress by activating the antioxidant defense system and other protective systems. Therefore, the aim of the present study was to determine the effect of intense endurance training and intense periodicity on co-oxygenase-1 and alpha tumor necrosis factor in the heart tissue of male Wistar mice. 

The present experimental study was conducted on 24 adult Wistar rats in 2019. After two weeks of identification and weight lifting, they were randomly divided into 3 groups: control, intense endurance training, Intense periodic exercise). The protocol of intense and intense endurance training included running on a treadmill for 8 weeks (5 sessions per week), standard water and food, and was freely available to the subjects. Forty-eight hours after the last training session and 4 hours of fasting, the subjects were dissected and tissue samples were collected. Subsequently, the concentration of HO-1 enzyme was measured by ELISA and TNF-α was measured by ELISA. The collected data were analyzed using Shapirovilk statistical tests, one-way analysis of variance and Tukey post hoc test. 

There was no significant difference in TNF-α levels after 8 weeks of strenuous endurance and high intensity interval training (P˃0/05). Furthermore, 8 weeks of strenuous endurance training and high intensity interval training had no significant effect on the amount of HO-1 enzyme in heart tissue (p <0.05). 

Since the levels of HO-1 and TNF-α in heart tissue did not change after 8 weeks of intense endurance training and intense intermittent training, it can be concluded that regular exercise, by creating a beneficial adaptation in the antioxidant system, they make oxidative stress more resistant.