Isolation and Identification of Protease Producing Bacteria Kocuriasp. Strain HR12coexistence of Avicennia Marina from West Coastal Area of Qeshm Island (KonarSiah)

Due to the economic importance as well as the fact that microbial proteases are stable at the desired pH and tolerate harsh conditions, this study was conducted to isolate and identify bacteria that produce protease enzyme symbiosis in mangrove trees. In this study, protease-producing bacteria coexisting with the roots and leaves of mangrove trees were collected from the west coast of Qeshm Island (Konar Siah) and cultured them at 30 °C for 72 hours on nutrient agar medium.. Protease-producing bacteria were then screened by specific culture medium containing Skim milk agar and protease enzyme activity of the isolated bacteria was measured using 1% casein substrate. Finally, the bacterium with the highest enzymatic activity was selected and identified by 16SrRNA gene sequence analysis. The results of the analysis showed that 31 of the 46 bacteria isolated from mangrove leaves and roots were capable of producing protease enzymes. Among the six strong bacteria, one with the highest enzymatic activity was selected, which after molecular processes and sequencing led to the identification of the bacterium Kocuria sp. strain HR12. Kinetic studies revealed that the protease enzyme isolated from kocuria sp. HR12, exhibited its maximum activity at 50 ˚ C and pH 9.. The findings of this study provide a platform for the mass production of temperature-resistant protease enzymes from the coexisting bacteria of the Persian Gulf mangrove trees, which offers a wide range of potential uses for practical enzyme production.