Effect of aerobic exercise on gene expression of Drp1 and Cyp1a1 and protein expression of CYP1A1 in the soleus muscle of male diabetic rats

Improper regulation of mitochondrial dynamics is associated with dysregulation of metabolism and can lead to metabolic diseases. This study aimed to investigate the effect of aerobic training on gene expression of Drp1 and Cyp1a1 and protein expression of CYP1A1 in soleus muscle of male diabetic rats. In this experimental study, 24 male Wistar rats were randomly divided into 3 groups: diabetes (D), control (Ct), and exercise + diabetes (ED) group. The diabetic model was induced in rats by STZ injection. The ED group performed an aerobic training protocol five times a week for eight weeks. After the end of the protocol, rat soleus muscle was extracted, and gene expression of Drp1 and Cyp1a1 RT-PCR and protein expression of CYP1A1 were measured using ELIZA. RNA was calculated using the formula 2^(-ΔΔCT)and then the data were analyzed using ANOVA. The results showed a significant difference between groups in the gene expression of Drp1 (P0.001) and Cyp1a1 (P=0.017), protein expression of CYP1A1 (P=0.028), and glucose (P0.001). Diabetes increases the gene expression of Drp1 and Cyp1a1 and the protein expression of CYP1A1 in skeletal muscle. However, exercise could only reduce gene expression of Drp1 (P=0.013). Diabetes leads to increased glucose, gene expression of Drp1 and Cyp1a1, and protein expression of CYP1A1 in skeletal muscle. But the exercise could prevent only increase of gene expression of Drp1.However, exercise could only reduce Drp1 expression (P=0.013). Diabetes leads to increased glucose, gene expression of Drp1 and Cyp1a1, and protein expression of CYP1A1 in skeletal muscle.