Isolation, Identification, Determination of Serotypes and Evaluation of Antibiotic Resistance of Poultry Salmonella in Khuzestan Province
Different species of Salmonella are important pathogenic bacteria in animals and humans that often infect poultry flocks. Most Salmonella infections in humans are caused by eating foods contaminated with this bacterium. The aim of this study was to determine the Salmonella infection status of several poultry farms in Khuzestan province and to determine the serotypes and antimicrobial resistance pattern of isolated Salmonella. In the year 1392 total of 1829 samples including stool swabs, litter samples, feathers and eggshells were collected from a broiler mother farm, two laying mother hen farms and an incubator and cultured in enriching and selective media and detected using biochemical tests according to standard methods. In this study, 10 Salmonella isolates were isolated including serotypes enteritidis (3 cases), corvallis (3 cases), typhimurium (3 cases) and ruzizi (1 case) using Salmonella serum type tests. After performing antibiogram test with standard disk diffusion method, it was determined that 100% of the isolates were resistant to Colistin, Cefalexin and Cefazolin. Isolates were highly resistant to Ceftazidime, Nalidixic acid, Ampicillin, Ciprofloxacin, Nitrofurantoin, Amoxicillin, Neomycin, Streptomycin, Kanamycin, Ceftizoxime, Gentamicin and Tetracycline. All Salmonella isolates in this study were sensitive to Amikacin, Enrofloxacin, Ceftriaxone, Imipenem, Cefixime, Cefotaxime, Ofloxacin, Cefepime, Piperacillin, Florfenicol, Furazolidone, Chloramphenicol and Trimethoprim- Sulfamethoxazole.
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