Association of rs13429458 and rs12478601 Single Nucleotide Polymorphisms of THADA Gene with Polycystic Ovary Syndrome

It is thought that genetic factors are influential in the etiology of polycystic ovarian syndrome (PCOS),the most frequent endocrinological disorder of females in their reproductive age. This study was carried out to elucidatethe association of rs13429458 and rs12478601 single nucleotide polymorphisms (SNPs) of the THADA gene andthe risk of the PCOS among a population of Iranian female patients. This case-control study contains 66 infertile women with PCOS (patient group) and 44healthy women without PCOS (control group) that referred to the IVF Unit of the Infertility Research Center of theAcademic Center for Education, Culture and Research (ACECR). The polymerase chain reaction (PCR) was utilizedto amplify genome DNA as well as direct sequencing to determine SNPs. The THADA rs12478601 and rs13429458genotypes were consequently examined with amplification refractory mutation system-PCR (ARMS-PCR). In this study, we observed that rs13429458 polymorphism was not associated with PCOS risk in two groups (P=0.42). On the other hand, data analysis indicated that the rs12478601 genotype significantly increased the risk of PCOS in the case group (P=0.032) in compared with control group. We found that the “T” allele of rs12478601 in the THADA gene had a significant relation to PCOS in the case group (odds ratio [OR]: 2.574, 95% confidence interval [CI]: 1.439-4.604, P=0.001). This study has presented further evidence that TT and CT genotype of THADA rs12478601 is associatedwith a high risk of PCOS.