Anti-nociceptive Activity of Quebracho tannin Extract on Pain Induced by Formalin and Writhing Tests in Mice

Based on positive role of the tannins for pain relief, there is no report for possible antinociceptive activity of the Quebracho tannin.

This study aimed to determine the anti-nociceptive activity of the Quebracho tannin extract (QTE) on pain in mice.

For this purpose, 340 mice were used for formalin and writhing tests each including 4 experiments with 4 sub-groups. In experiment 1, mice were injected with saline, QTE (100 mg/kg), QTE (200 mg/kg), QTE (400 mg/kg), and morphine (5 mg/kg). In the second experiment, injections included saline, QTE (400 mg/kg), naloxone (2 mg/kg), and QTE + naloxone. Experiments 3 and 4 were similar to experiment 2, except that mice injected were with NG-nitro arginine methyl ester (L-NAME, 10 mg/kg) and cyproheptadine (4 mg/kg) instead of naloxone. Then, formalin (1%) was injected, and time spent for licking the injected paw was recorded until 30 minutes following injection in the first and second phases. Finally, injections in 4 experiment groups were the same, and animals were intraperitoneally injected with acetic acid, and contractions were recorded in the writhing test category.

According to the results, QTE (100, 200, and 400 mg/kg) decreased pain in the injected paw (P=0.001) and inhibited the pain response by 59.37% (P=0.001). Moreover, the injection of naloxone + QTE significantly decreased pain in the injected paw (P=0.021). Eventually, the injection of the L-NAME + QTE significantly reduced the anti-nociception effect of the QTE on the formalin test (P=0.031) and writhing contractions (55.75%, P=0.033).

These findings suggested anti-nociceptive properties of the QTE mediated by opioidergic and nitrergic systems.