Determination of Phenolic Compounds and Free Radical Scavenging Activity of Microalgae Spirulina platensis by Various Extraction Methods

In this study, extracts of microalgae Spirulina platensis were extracted by maceration, microwave and ultrasound methods and water and ethanol as solvent under controlled conditions. Determination of total flavonoid content (TFC) and total phenolic content (TPC) as well as free radical scavenging activity (IC50) of the extracts done based on Alcl method, follin-ciocaltuea and absorption of radical DPPH (2,2-diphenyl -1-ipicryl hydrasil) respectively and in triplicate. The results showed that between extraction techniques, ultrasound and between two solvents used, water had the best efficiency of extraction the TPC, TFC and free radical scavenging activity. By ultrasound technique, PC, TFC and IC50 were 2.13±0/36 (mg/g), 3.4±0/35 (mg/g) and 0.34 ±0/35 (µg/ml) respectively. As a conclusion, it can be concluded that the application of ultrasound technique and water in comparsion with other five treatments showed the best efficiency and finally, it was selected as an optimal treatment.