The effects of a mixture of nano selenium, vitamin C and E on growth indices, liver enzymes and nonspecific immune response in juvenile Huso huso

In this study, the effects of mixture of dietary nano-selenium, vitamin C and E on growth indices, liver enzymes and nonspecific immune response of juvenile Huso huso were investigated. One hundred and twenty individuals juvenile Huso huso with average weight of 77.77 ± 2.3 g were fed with four diets including treatment A (TA by adding 0.1, 30 and 100 mg/kg), treatment B (TB by 0.2, 60 and 200 mg/kg), treatment C (TC by 0.3, 90 and 300 mg/kg) and control group (without adding nano-selenium, vitamin C and E) for 10 weeks. At the end of the rearing period, blood samples were taken from fish to evaluate liver enzymes and immune system indices. No significant differences were observed in growth indices, feed conversion ratio and protein efficiency ratio (p>0.05). The lowest activities of ALT and AST were found in TA and TB (p>0.05). However, IgM and ACH50 levels were significantly higher in TB and TA than in control group, respectively (p<0.05). The results of this study indicated that the addition of a mixture of nano-selenium, vitamin C and E in TA and TB enhanced nonspecific immune system in juvenile Huso huso.