Determination of Rutilus kutum kisspeptin and its effect on reproductive hormones

The Caspian kutum (Rutilus kutum) is the most important commercially and nutritionally bony fish, which is used to prevent the extinction of this valuable species by induced reproduction to synchronize and increase the level of fecundity in breeding conditions. Previous research has shown that kisspeptin, a super-stimulatory protein in the sex-secreting gland axis, leads to puberty and gametogenesis. However, the sequence of kisspeptin 1 and 2 of R. kutum has not been determined and the ability of this peptide from protein (Kiss1) in reproduction has not been studied so far. In the present study, the kisspeptin10 sequence of R. kutum was determined by CLUSTALW method by designing a primer from the Kiss1 sequence of the same family fish of R. kutum. Doses of 5, 25, 50 and 100μg/kg were used to achieve the best dose of this peptide; and concentrations of 17-α hydroxy progesterone and 17-β estradiol were examined. The results of this study showed that synthetic kisspeptin 10 with an optimal dose of 100μg/kg can be introduce as a suitable candidate for induction of spawning in R. kutum.