Evaluation of the effect of eugenol essential oil on Trichomonas galine protozoan in vitro

Trichomonas galline is a flagellate parasite of warm-blooded animals found in birds that is more perceptive and lunar. Other birds may also be infected. In this study of pigeon stratigraphy, the British killed broth in a diamond environment. After culturing the parasite, the samples were kept in an incubator at 37 ° C for 48 hours until the number of protozoa reached about 1000 parasites per ml of culture medium. Then in eight groups including one control and seven treatments (one metronidazole group and six treatments of eugenol essential oil) according to doses of 1.56, 3.125, 6.25, 12.5, 25 and 5 micrograms per liter, eugenol is used to create the desired dilutions to the culture medium. . . After placing the microplate in the incubator at a temperature of 37 degrees at the desired intervals of 1, 2, 6, 12 and 24 hours after culture, 20 microliters of each well was removed and with 20 Landa trypan paint. It was mixed with the lamellar corner and after 10 minutes the number of dead Englishmen compared to the living ones was counted. Mortality in the metronidazole group showed a significant difference at all times of the study (p≤ 0.05). At 1.56, 3.125, 6.25, 12.5, 25 and 50 μg / ml eugenol, the difference between the mortality rates at 6 and 12 hours was significant for all of them (p≤ 0.05). The difference between mortality at 1 and 2 hours at 1.56 μg / L eugenol was not significant. The results of the study fully indicate the appropriate effect of eugenol extract in high dilutions (untreated (1.56 μg / ml)) on trichomoniasis.