Study on Sara-CoV 2 reserviors by phylogenetic analysis of aminoacid Sequences of S and Angiotension-converting Enzyme (ACE2) proteins

Sars-Cov2 has spherical shape with many spike proteins viz, M,N,E and S. S protein of this virus has major role in attachement of the virus to host receptor Angiotension-converting Enzyme (ACE2) protein.The purpose of this study was evaluation of Sars-Cov2 reservoirs by phylogenetic analysis aminoacid sequences of S and Angiotension-converting Enzyme (ACE2) proteins in the differents animals. To perform the study whole genome and amino acid sequences of S protein of Sars-Cov2 and sequences of ACE2 protein of some animals and human were downloaded from NCBI and GenBank and subjected to phylogenetic analysis of their relationship using MegaX soft ware. The results obtained indicated that all Sars-Cov2 isolated from different geographical areas have similar origin. On the other hand, amino acid profile of S protein in the different viral variants showed several sequences. In addition, amino acid sequence of Human ACE2 was very close to camel, Swine and bats (Pteropus vampyrus and Cynoplerus phinx). Therefore the present study showed that gene mutation in surface protein coding sequence of corona virus and the similarity of ACE2 amino acid sequences in different animals cause emerging the new variants of corona virus in the different reservoirs. Hence it might increase the rate of transmission of the virus to human even after vaccination and herd immunity.