Evaluation of the effect of gold nanostructure on the production of bio surfactants from Pseudomonas aeruginosa

Raminolipids belong to the group of glycolipid bio surfactants and were first isolated from Pseudomonas aeruginosa. Rhamnolipids are a good alternative to synthetic surfactants due to their low toxicity, biodegradability and selective performance. In this study, the effect of gold (Au) nanoparticles on the growth and production of Pseudomonas aeruginosa PBCC5 bio surfactant was evaluated. Different concentrations of 1, 500 and 1000 mg / l nanoparticles were used. In this study, bio surfactant surface tension and emulsification indices (24E) were measured. The presence of nanoparticles on the bacterial surface was investigated by TEM and the morphology of nanoparticles was investigated by SEM. Binding of nanoparticles to bio surfactants was confirmed by TEM. The results showed that gold nanoparticles were not bactericidal and also increased bacterial growth and bio surfactant production. The surface tension of all samples was reduced from 72 mN / m distilled water to 35-32 mN / m.