The effect of plant growth regulators on induction and callus growth in four grape cultivars with the aim of resveratrol extraction

Plant callus tissue has a variety of capabilities, including its use in the preparation of cell suspension cultures to produce secondary metabolites and beneficial natural compounds. In order to induce callus in four varieties explants of Rajabi Sefid Shiraz, Bidaneh Ghermez, Shahani and Makr Quchan from two hormonal compound groups of 2,4-D (in three levels) with BA and NAA (in three levels) with Kinetin were used. In most explants, the highest percentage of induction was observed at the third level of naphthalene acetic acid, which did not show a significant difference with the second level. The response of explants to different levels of plant growth regulators was different and dependent on cultivars, in induction and proliferation. Concentrations of 0.7 mg / l NAA and 0.2 mg / l Kinetin were selected to obtain cell suspension cultures, which good quality was obtained by several subcultures. After finding the appropriate concentration and composition of the hormone in the culture medium, the aim of this study was investigation of the diversity of Resveratrol cell production ability in different grape varieties under cell suspension culture. Measurements of the Trans-Resveratrol content of cell suspension cultures showed that the highest Trans-Resveratrol content was related to the cell suspension culture of Rajabi Sefid Shiraz. Differences in the genetic information of cultivars have been introduced as one of the most important reasons for differences in resveratrol content in grapes.