Effects of flavonoid compounds in walnut green skin on increasing cell therapy efficiency by differentiating adipose-derived mesenchymal stem cells into insulin-producing cells in diabetic rats

In this study, investigated the effect of flavonoid compounds in walnut skin on the production of insulin-secreting cells.

In this experimental study, adipose-derived mesenchymal stem cells (AD- MSCs) were differentiated into insulin-producing cells under sterile conditions for 21 days in the presence of flavonoid extract at doses of 50 and 100 mg/ml. Streptozotocin at a dose of 60 mg/kg was used to diagnose rats. Dithizone staining (DTZ) for insulin, Immunofluorescence to determine the presence of pancreatic beta-specific proteins, Blood glucose measurement by glucometer, Serum creatine, urea and uric acid levels by calorimetry and Reverse chain reaction transcription E- polymerase (RT-PCR) was used to evaluate the expression of PAX4 gene.

AD-MSCs under the above conditions gradually changed from spindle-shaped fibroblasts to circular cells and showed insulin-producing cells using red DTZ dye and insulin secretion .Expression of insulin-pronsulin and beta receptor markers was demonstrated, blood sugar, urea, uric acid and creatinine levels decreased and the expression level of PAX4 gene in experimental groups showed a significant disorder (P≥0.05).

The results showed that AD-MSCs under the flavonoid extract of walnut green skin have the ability to differentiate into insulin-producing cells. Keywords: Mesenchymal stem cells, Insulin-producing cells