Molecular identification of Fusarium species associated with turfgrass and some trees in Ardabil greenspace

Among the various diseases that threaten the green space plants, the Fusarium genus is of great importance. Due to time consuming, requiring high expertise and low reliability in the morphological methods, it would be recommended to use species-specific primers for identification of Fusarium species as a fast and cost-effective method without need to sequencing that could be easily used to identify the genus and species of Fusarium. In this study, in order to molecularly confirming of Fusarium isolates associated with Ash, Black locust and Populus decline, and turfgrass yellowing, in addition to verifying species-specific primers with standard isolates, Fusarium isolates obtained from Ardabil’s green space plants were identified using these specific primers by PCR. Totally, the 24 isolates belonging to F. solani using fFuso1 and rFuso2, 11 isolates belonging to F. oxysporum species using CLOX1-F and CLOX2-R primers, 10 isolates belonging to F. acuminatum species with using FAC-F and FAC-R primers, 8 isolates belonging to F. equiseti were identified using FEF1 and FER1 primers and 8 isolates belonging to F. graminearum were identified using FgCTPSf024 and FgCTPSrR306 primers. These primers did not react with isolates belonging to other Fusarium species. Also, the sequencing and phylogenetic analysis results lead to identify 2 and 1 isolates as F. solani and F. redolens, respectively.