Development and laboratory scale production of inactivated triple ND+AI (H9N2)+IBD vaccine

Newcastle disease (ND), avian influenza (AI) and infectious bursal disease (IBD) cause severe damage to the poultry industry. Vaccination is often used to prevention or control the spread of poultry diseases. Inactivated vaccines are used to produce the uniform antibody titer, the increase antibody levels and its shelf life. In this study, the ND, AI and IBD triple inactivated vaccine was designed and prepared in laboratory scale. Then its immunization potential was evaluated in SPF chickens. To produce the triple vaccine, the ND V4, avian AI H9N2 and IBD 07IR standard strains were separately propagated in embryonated eggs. The viral suspensions were inactivated after virus titration. Different amounts of each virus were considered as a dose and introduced as a vaccine after standard control tests. The prepared vaccine was injected along with an imported vaccine to different groups of SPF chickens and HI, VN and ELISA tests were performed on serum samples. The HI titer was 7.6 for ND and 6.7 for AI. In the VN test, the neutralization index for IBD was 3.5. In the ELISA test, the antibody titer against ND was 8481, for AI 7569 and for IBD 3108. These data did not differ significantly from the results of the imported vaccine. The results showed that the developed triple inactivated vaccine provided good immunity and also had no side effects for SPF chickens.