Calibration and performance evaluation of ultraviolet-visible spectrophotometer

Spectrophotometer is one of the instruments which is vastly used in variety of chemical and biochemical tests in laboratories. Performance qualification and reproducibility of this instrument can affect the different tests results. Validation or qualification of an instrument is a process during which suitable performance of the device and its compliance with intended purpose is proven by documentation. Calibration of a device is one of the most important parts of its qualification.Calibration of spectrophotometer consists of some steps including the evaluation of accuracy, precision and likely error of the instrument. This article describes the calibration of Spectrophotometer using the control of wavelengths, absorbance, and limit of stray light and equality of cuvettes. The most important parameters in the calibration of the visible-ultraviolet spectrophotometer are the control of the absorbance and also the control of the wavelength generated by the spectrophotometer. Control is done by cells or reference materials and comparing between the values created by the device and true values.