Evaluation of NAC10 and NAC6 gene expression on Transgenic and Non-Transgenic Rice

The proteins that bind to the elements that respond to drought stress, have particular important in rising plant resistance to drought. In this study with the aim of evaluate different expression of TFs in different tissue, the expression of genes encoding NAC10 and NAC6 transcription factors in rice under drought stress in leaf and anther tissues were estimated. In this study, transgenic and non-transgenic rice seeds of Nipponbare variety were cultivated. Leaf and anther tissues on young microspore stage were sampled at several times after Stop irrigation. Promoter analysis for finding transcription binding sites has done by two web sites and two TF from NAC family were selected. RNA extraction was maintained at -80 ° C, RNA quality and quantity were evaluated. Expression comparison of genes encoding these transcription factors were performed by real-time PCR with Delta-Delta CT method. The results of this study showed that NAC10 and NAC6 are expressed in both anther and leaf tissues. Under drought stress, the highest expression of both transcription factors was in anther tissue after 24h stress, the highest expression of NAC10 was in non-transgenic plants, about 70 times more than control and the highest expression of NAC6 was in transgenic plant about 60 times more than control condition. These results indicate the important role of these factors in anther tissue of rice, response to drought stress.