The effect of endurance and high-intensity interval training on the content of mTOR, CRTC1 and CRTC2 proteins in subcutaneous adipose tissue of type 1 and 2 diabetic rats

Many cellular and molecular factors are involved in the regulation of adipose tissue metabolism, which diabetes can lead to their functional impairment. Therefore, the aim of the present study was to evaluate the effect of endurance and high-intensity interval training on the content of mTOR, CRTC1, and CRTC2 proteins in subcutaneous adipose tissue of type 1 and 2 diabetic rats.

In this study, 36 head of 2-month-old Sprague-Dawley male rats with a mean weight of 280±30 g were selected. After induction of type 1 (18 head) and 2 (18 head) diabetics through streptozotocin and nicotinamide solution, each type of diabetes was randomly divided into 3 groups: endurance training, HIIT, and control (6 heads per group). The training groups performed endurance training and HIIT program 4 days a week for 4 weeks. The content of the present study variables in subcutaneous adipose tissue was measured by in vitro Western blotting method. SPSS software version 23 and One-way ANOVA and Tukey post hoc tests were used to analyze the data.

Endurance training and HIIT led to a significant increase in the protein content of mTOR (P=0.0001), CRTC1 (P=0.0001), and CRTC2 (P=0.0001) in subcutaneous adipose tissue.

It seems that four weeks of endurance training and HIIT can regulate subcutaneous adipose tissue in type 1 and type 2 diabetic subjects by increasing the proteins of the present study.