The histochemical and histological survey of female Mullie liver and ovary (Pociliae sphenops) on three groups (adult, preadult and immature)

This research was performed in order to survey the histochemical and histological factors of female Mullie  ovary and liver ( Pociliae sphenops)on three groups(adult, preadult and immature) in 2008 at laboratory complex of Science and ResearchUniversity. Then 10 numbers of each group(totally 30) were evaluated to determine the histochemical factors(liver and ovary protein and lipid). The tissues were kept in -70 ċ to do the rest of experiments. Also 12 numbers were surveyed to do the histological process and observation by light microscope(Hematoxilin-Eosin, Von- kossa for calcium and Sudan- Black B for lipid). According to statistical analysis, protein and lipid percentages of ovary tissue on three groups, adult(4-month-old), preadult(3-month-old) and immature(1.5- month-old) revealed the significant variation(P<0.05) and immature, preadult and adult groups had respectively the most and least lipid and protein percentages of ovary. The significant variations (P<0.05) also were seen by liver protein and lipid. The immature livers protein was higher than preadults and adults. The measurement of liver total lipid showed that preadult lipid was more than adult and immature. Calcium staining in both liver and ovary tissues expressed that this element will increase during growth and development.