Evaluation of antimicrobial effects and estimation of anti-cancer potential of green nanoparticles biosynthesized using hyssop extract on different cell lines (A549, MCF-7 and Hela)

Metal nanoparticles synthesized using medicinal plants are used in anti-cancer and antimicrobial studies due to their unique physicochemical properties, high safety and low cost.

Silver nanoparticles were synthesized using extracts of hyssop and marjoram. After studying the properties of the synthesized nanoparticles, two gram-negative Escherichia coli and gram-positive Staphylococcus aureus bacteria were used to evaluate the antimicrobial effect of the synthesized nanoparticles. Finally, the anti-cancer effect of synthesized silver nanoparticles on 3 cancer cell lines was evaluated by MTT method.

The results showed that the synthesized nanoparticles were spherical in shape and their average size was between 15 and 26 nm. X-ray diffraction test also confirmed the successful synthesis of nanoparticles. The results of antimicrobial test showed that the synthesized silver nanoparticles had a significant antimicrobial effect on both bacteria. The anti-cancer effect of the synthesized nanoparticles was similar on all 3 cell lines. However, with increasing the concentration of nanoparticles on the survival of cancer cells decreased, indicating a direct dose interaction on the inhibitory rate of silver nanoparticles. Discussion and

Medicinal plants can be used in the synthesis of silver nanoparticles. The synthesized nanoparticles, due to their coating of secondary metabolites and the release of silver ions (Ag+ ), can be used as effective therapeutic agents in the treatment of various cancers