The effect of aquatic extract of canola (Brassica napus L.) on chlorophyll content, nitrate reductase, catalase and peroxidase activities enzymes of soybean (Glycine max L.) seedling in hydroponic culture

Canola contains allelochemicals that effect on metabolism of weeds and crop plants.The aim of this research was to study the effect of aquatic extract of canola (Brassica napus L. cv Hyola 401) on chlorophyll content in cotyledon, nitrate reductase, catalase and peroxidase activity in root, stem and cotyledon of soybean seedling (Glycine max L. cv Gorgan 3) in Hoagland culture. The seeds of canola (Brassica napus L.cv Hyola 401) was planted in pot and provided aquatic extract of total plant in 5 leaf stage.This extract was added to Hoagland culture and catalase,peroxidase and nitrate reductase activities in leaf, stem and root and chlorophyll a and b content in cotyledons in soybean seedling (Glycine max L.cv Gorgan3) after 9 days were evaluated. The results showed that chlorophylls (a and b) amounts in cotyledons and catalase and peroxidase in root and stem and cotyledon decreased in comparison with to control (Hoagland only) in while nitrate reductase activity reduced in cotyledon and in root and stem increasd.