Experimental Study Extraction Active Ingredients of Eryngium Billardieri Using Supercritical Carbon Dioxide

Recently, supercritical fluids have been introduced as a suitable environment for extraction. Supercritical carbon dioxide is one of the most commonly used supercritical fluids due to its cheapness, toxicity, inseparability, environmental compatibility, and extraction operations at low temperatures. Low critical temperatures of carbon dioxide have led to its widespread use as a suitable solvent for temperature-sensitive substances such as food and pharmaceuticals. In this study, using supercritical carbon dioxide, extraction of active compounds of Eryngium Billardieri was investigated. In this regard, experiments were conducted on operating the pressure (110, 130 and 150 times), temperature (35, 40 and 45 °C), time (30, 50 and 70 minutes), and the particle size (0.075, 0.1, and 0.2 mm) using Taguchi design in Minitab software. The highest efficiency was obtained under operating conditions of 40 °C, the pressure of 150 bar, the particle size of 0.075 mm, and the time of 50 minutes. Also, using GC-MS test data, the most synthesized material is Di Iso Octyl Ester.