Structural and morphological investigation of polycaprolactone multi-component scaffold containing collagen microsphere/bioglass nanoparticles

Bioglass nano-ceramic containing cerium (Ce) with various amounts of Ce were synthesized by sol-gel method for enhanacement polycaprolactone (PCL) fiber scaffolds for tissue engineering approaches. The prepared scaffold was analysize using scanning electron microscopy (SEM) and X-ray diffraction (XRD) to characterize the synthesized ceramic powder. The obtained results indicated proepr doping of Ce inside the bioglass microstructure. The sample were successfully encapsulated inside collagen microspheres by water-in-oil emulsion technique, an average particle size and hydrodynamic diameter of microspheres were determined by SEM images and DLS technique, respectively. The samples were submerged in the simulated body fluid (SBF) indicated that all PCL/collagen microspheres/bioglass58S/cerium fiber scaffolds have acceptable bioactivity to form apatite. In this study, the MG63 cell line cultured on PCL fiber scaffold/collagen microspheres/bioglass 58S/cerium which indicated 3% of its potential for hard tissue approaches. The obtained results indicated that after 7 days, the rate of cell proliferation and growth increased compared to the initial days (except for PCL fiber scaffold containing collagen microspheres/bioglass 58S nanoparticles/10% cerium (mass-mass) after 7 days.