Effect of Salix Alba Leaves Hydro-Alcoholic Extract on PTZ-Induced Seizure in Male Mice

Regarding the chronic nature of epilepsy and resistance to chemical drugs, herbal medicine has received remarkable interest. This study investiagted the anticonvulsant effects of hydro-alcoholic extract of Salix Alba leaves on PTZ-induced seizure in male mice.

In total, 56 albino male mice weighing 20-25 g were divided randomly into seven groups (8 animals per group) including negative (saline), positive (Phenobarbital) control, and five groups which received different doses of hydro-alcoholic extract of Salix Alba leaves. After 45 min, all groups received the convulsant pentylene tetrazole, and the onset time of myoclonic, tonic-clonic seizures, and the 24-hour mortality rate were measured.

The extract significantly delayed the onset of myoclonic and tonic-clonic seizures at a dose of 100 mg/kg (P<0.01) and for other doses (P<0.001), compared to the control group. Moreover, the extract decreased the 24-h death. This was significant at 1000 mg/kg (P<0.001), as well as 300, 500, and 800 mg/kg (P<0.05), compared to the control group.

It seems that the hydro-alcoholic extract of Salix Alba leaves has a significant diminishing effect on PTZ-induced seizures in male mice which is due to its anti-inflammatory and anti-oxidant effects.