Evaluation of Effect of Carbon Nanoparticles on the Proliferation of Calli of date palm (Majol cultivar)

Nanotechnology, as a promising method for addressing sustainable agricultural issues, can increase propagation efficiency in palm tissue culture. The aim of this study was to prepare carbon nanoparticles and use the resulting nanocomposites to evaluate their efficiency in improving and increasing date callus formation.

Three separate experiments were performed to propagate calluses consisting of meristematic microsamples of dates. In the first experiment, calli prepared in MS culture medium were transferred to four culture media with different hormonal treatments from NAA and 2iP, and in the second experiment, calli composed of meristematic date microsamples in MS culture medium were transferred to four culture media with separate treatments from NAA and BAP. Different hormones were transferred from NAA and BAP. In the third experiment, after determining the best hormonal treatments from the first and second experiments, carbon nanoparticles were synthesized from graphite and calli composed of meristematic date microsomal samples were recreated in superior culture media with different concentrations of nanoparticles (0, 10, 20, 30, 40, 50 mg/L).

Based on the results of the first experiment, treatments of 10 mg / l NAA + 30 mg / l 2ip and 0.1 mg/l NAA + 0.05 mg/l 2ip were selected as the best callus propagation treatments. In the second experiment, it was found that there was no statistically significant difference between the applied treatments of 10 mg/l NAA + 30 mg/l BAP and 10 mg/l NAA + 10 mg/l BAP with other treatments with different concentrations of BAP. The results of the third experiment showed that the use of 10 mg/l NAA + 30 mg/l BAP + 30 mg/l CNP can produce the most calluses.

Due to the positive effect of carbon nanoparticles on increasing the weight of commodities, treatment of 10 mg / l NAA + 30 mg / l BAP + 30 mg / l CNP becomes the most suitable option for calorific cultivar propagation.