The effect of endurance training on gene and protein expression of TGFβ and integrin in the liver of male rat diabetic

Diabetes is a syndrome of metabolic that courses of chronic hyperglycemia, stress oxidative and inflammation. The aimed this study was to investigate the effect of endurance training on gene expression of transforming growth factor Beta (TGFβ) and integrin in the liver of male rat diabetics. In this experimental study, 32 male Wistar rats were randomly divided into 4 groups of 8 per groups: diabetes (D), control (C), exercise (E) and diabetes and exercise (ED). Then 16 rats got infected with diabetes by STZ injection. Groups E and ED performed aerobic exercise protocol five sessions per week for eight weeks. Then, liver tissue was extracted, and gene expression were measured using RT-PCR and protein expression of TGFβ and integrin were measured using immunohistochemistry and western blotting methods respectively. RNA was calculated using the formula 2-δδCT and the data were analyzed using ANOVA. The results showed that the gene and protein expression of TGFβ in group D was significantly higher than group C and the protein expression of TGFβ in group ED was significantly reduced compared to group D but was still significantly higher than group C (P<0.001). Also in group E, TGFB protein expression decreased significantly compared to group C (P<0.001). Gene expression of integrin was significantly higher in groups D and ED than group C and its protein expression were significantly lower (P<0.001). Finally, the findings showed that early in diabetes, increased TGFβ protein expression in liver tissue and decreased integrin and endurance training reverses these changes.