The Effect of Crop Rotation on the Diversity and Abundance of Plant Parasitic Nematodes in Potato Fields

In order to identify the plant parasitic nematodes of potato fields in Western and central regions of Isfahan province, 45 soil and root samples were collected from rhizosphere and aerial parts of potatoes during 2018 and 2019. The nematodes were extracted by combined sieving and centrifugal-flotation method of Jenkins and transferred to glycerin by using DeGrisse method. The permanent slides were prepared and the nematodes were studied by light microscopy. In this research, 20 species from nineteen genera belonging to Tylenchina and Aphelenchina suborders were identified as follows: Amplelimerlinius sp., Aphelenchoides limberi, Aphelenchus avenae, Boleodorous thylactus, Ditylenchus destructor, Filenchus filiformis, Filenchus polyhypnus, Haplolaimus galeatus, Helicotylenchus vulgaris, Irantylenchus sp., Merlinius brevidens, Neopsilenchus magnidens, Paratylenchus similis, Pratylenchoides ritteri, Pratylenchus neglectus,   Psilenchus iranicus, Rotylenchus iranicus,  Scutylenchus rugosus, Tylenchus davainei, Zygotylenchus guevarai. The species Helicotylenchus vulgaris, and Irantylenchus sp. were measured with 58 and 2 percent frequency, respectively. In this research, the asterisk species were the new records for potato nematode fauna of Isfahan province.