Immobilized Peptide on the Surface of Core-shell LaVO4: Eu3@poly (levodopa) for Targeted Delivery of 5-Fluorouracil to Breast Tumor

Chemotherapy using drug delivery systems can target tumor cells selectively and do not affect normal cells. In this study, a specific drug delivery system was designed with immobilized NL2 peptide on the surface of polymeric nano drug for breast tumor treatment. The tertiary structure of NL2 peptide (AEGEFIHNRYNRFFYWYGDPAK) was selected from the database and synthesized. After that, it was coupled to the synthesized poly-3,4-dihydroxy-1-phenylalanine (DOPA)/SiO2 nanocomposite and tested for targeted delivery of 5-fluorouracil (5-FU) to SK-BR3 breast cancer cell line and MCF-10A normal breast cell line. The results of MTT and hemolysis of red blood cells showed that the components of the nanocomposite and NL2 peptide do not have any cytotoxicity, while the results of the MTT test after washing showed that the nanocomposites functionalized with NL2 peptide had specificity for cells with Her2 marker, i.e. SK-BR3 cells, but after washing, they were separated from MCF-10A cells, which lack this marker, and the drug 5- Fluorouracil cannot exert its toxic effects on these cells.