The Effect of Eight Weeks of Aerobic Exercise on AT and TT Genotypes, rs1870377 Polymorphism of VEGFR Gene and Changes in Aerobic Performance of Untrained Women

The present study was conducted with the aim of investigating the effect of eight weeks of aerobic training on the AT and TT genotypes of the VEGFR gene polymorphism rs1870377 and the changes in aerobic performance of untrained women. For this purpose, 29 inactive women aged 30 to 45 were randomly selected from volunteer women in Shahriar, Tehran Province. The Subjects did 8 weeks of aerobic training in 5 sessions per week and each session lasted 30 minutes with an intensity of 55 to 75% of reserve heart rate. In the first two weeks, they trained with 55-65% of the maximum heart rate, and in the second two weeks with 60-65% of the maximum heart rate, and in the last 4 weeks with 65-75% of the maximum heart rate. 10 minutes for warming up and 10 minutes for cooling down were considered in each training session. Bruce's seven-step test was used to determine the VO2max before and after the exercises. The test started with a slope equal to 10% and a speed of 2.7 km/min on the treadmill, and each stage was completed in three minutes, so that in the seventh stage, the slope was 22% and the speed was 9.6. Then, among the subjects who were able to perform the desired test based on the researcher's expectation, saliva sampling was done for DNA sequencing to determine the genotypes. The RFLP method was used to determine the gene genotype. Enzymatic digestion at 65 degrees Celsius overnight, including one microliter of enzyme, 3 microliters of PCR product, 2 microliters of special buffer and 15 microliters of deionized water was used. The results of data analysis were analyzed using the dependent t test. The results showed that VO2max in women with AT genotype was not significant before and after exercise intervention (p = 0.840). The amount of VO2max in women with AT genotype was not significant on average before and after exercise intervention (p = 0.633). Also, the amount of VO2max in women with AT genotype, after exercise intervention, was not significant compared to TT genotype. In this research, it was shown that the significant improvement of Vo2max did not depend on their genotypic differences, and between AT and TT genotypes, the rs1870377 polymorphism of the VEGFR gene and the changes in aerobic performance of obese untrained women after eight weeks of aerobic training no significant relationship was observed.