Variation of rhizobium bacteria isolated from the ribosomal nodes of the root of alfalfa (Medicago sativa) plant using 16rRNA gene shear fragments

The alfalfa (Medicago sativa) plant was collected from the area of Andimeshk and in the spring of 2018. To express the 16S rDNA gene, the isolated bacteria were cultured in YMA environment under aerobic conditions and at a temperature of 25-28 °c. Genomic DNA localization was performed using the DNA extraction kit of SINA Gene Co. General and complete primers of sinobium bacteria were extracted from the NCBI site based on 16s rRNA gene sequences, and were used in sequencing the isolated bacteria genes in this study. The sequencing obtained by using Chromas Pro software was edited and compared using blasting software in NCBI database of three strains of alpha-alpha (Medicago sativa) were isolated and identified. The microscopic and macroscopic studies of the bacteria were identified. Bacterium 1 was identified as Gram-negative and caused by cream and embossed colonies, the second Gram-negative bacteria, with red-colored colonies and third bacterial colony isolated from the genus Alfalfa (Medicago sativa) plant. Sequencing results of 16S rRNA strains isolated from the Rhizoum species (Sequence ID = CP 021215.1) Sinorhizobium Meliloti (bacter1) (overlapping percentages 97 and game chats 2 percent), the second bacterium Sequence ID = CP-000738.1)) Sinorhizobium Medicae (bacteria 2) (the overlap percentage of 97 and 2-percent game chats) and third bacteria (Sequence ID = DQ-145546.1) Sinorhizobium meliloti (overlapping percentages 04.96 and 4% game chats) were identified.