Differential Expression of StFtsH5, StFtsH6, and StFtsH10 Genes in Potatoinduced by Cold, Heat, and High Light Stress

Abiotic stresses are among major factors limiting crop yields. Cold, heat, and high-light are the most important abiotic stress among plants. Thus, it is considered the understanding of molecular mechanisms response to stress.  FtsH metallopeptidase is one of the key regulators of plant response to abiotic stresses. Many surveys were performed on the FtsH in different plants. However, there is not report on potato under cold, heat, and high-light stress.

In this study, the gene expression of StFtsH5, StFtsH6, and StFtsH10 was evaluated in root, stem, and leaves under cold, heat, and high light stresses. Agria genotypes were planted under controlled greenhouse conditions. After cold, heat, and high light treatment duration, leaves, stem, and root sampling were performed under two conditions stress and normal after 24h and 48h treatment. The relative expression of selected genes was estimated.

StFtsH5 gene was strongly up regulated in leaves and stem at the 24h and 48h under cold stress as compared to non-stress condition, respectively. Further, this gene had the higher mean relative expression than normal condition in root and leaves at the 24h and 48h under heat stress. At 24h after high light stress, the more mean relative increase StFtsH5 gene than control condition was observed. However, StFtsH6 gene is not increased gene expression under low temperature, and high light after utilization of stress treatment in leaves, stem, and root. StFtsH10 gene showed the significantly increased expression of in stem and root under high-light stress at the 48h. It showed an increase in gene expression in stem and leaves under cold stress at the 48h after stress treatment. However, this gene had the high gene expression in stem and root as compared to control at the 24h and 48h after stress treatment. The results of this study showed that StFtsH5 and StFtsH6 had the maximum and minimum gene expression in stem, leaves, and root under three stress treatments.

Among three genes, StFtsH5 showed the high expression in three tissues under cold, heat, and high-light stress. According to the above results, changes in gene expression of StFtsH5 could refer to the key roles of this gene in abiotic stresses, particularly cold, heat, and high light stresses.