The effect of priming with Ellagic acid on the seed reserves mobilization and the growth of soybean seedlings under accelerated aging

In order to investigate the effect of ellagic acid on the growth of seedling and the qualities of soybean in accelerated aging conditions, a pilot was designed in 2019 in the research laboratory of the Shahrood University of Technology. The treatments included aging seed in two levels (unaged seeds and aged seeds) and priming at seven levels (control, distilled water, 25, 75, 50, 100, 125 mg/L with ellagic acid). A pilot study as a factorial experiment with a completely random-base design (CRD) in three replications was carried out under a germinator environment in accordance with standards. To prepare aged seeds in the laboratory, the seeds were incubated at 41°C and 95% relative humidity for 72 hours. Seed priming was soaking with ellagic acid for 6 hours in accordance with the principles of seed aeration. The aging reduced Vigor Index, Allometric Growth Ratio and Seed Reserves Use Rate. The increasing of the malondialdehyde had a negative effect on germination percentage through the influence of Vigor Index and α-amylase activity enzyme. The application of ellagic acid increased the germination percentage in unaged seeds and aged seeds through increasing the activity of α-amylase activity enzyme. The priming of seeds with ellagic acid reduced the malondialdehyde and electrical conductivity. Based on the results of research, the application of 50 mg/L of ellagic acid can be introduced as the best concentration of this material. It can also be suggested that the use of ellagic acid as a seed priming improves the effects of seed aging on soybeans.