EFFICACY OF INTRAUTERINE INSTILLATION OF AUTOLOGOUS PLATELET-RICH PLASMA ON ENDOMETRIAL GROWTH DURING THE FROZEN-THAWED EMBRYO TRANSFER CYCLES

Interventional studies about platelet rich plasma efficacy in the patients with Repeated Implantation Failure (RIF) are not sufficient, while RIF is one of the major limiting factors in treating infertile patients. Therefore, the current study was designed to evaluate whether autologous intrauterine platelet rich plasma (PRP) instillation improves endometrial thickness (ET) in RIF patients during in vitro fertilization-embryo transfer (IVF-ET).

In this interventional study, a total of 20 women were enrolled whose embryo transfer cycles were canceled due to lack of proper growth of the endometrium or repeated implantation failure. In the study subjects, PRP was administered intrauterinely, and ultrasound was performed again 72 hours after the injection and the thickness of the endometrium was checked with Doppler ultrasound. Data were analyzed using SPSS 18 software.

The mean ET was calculated 6.80 ± 1.46 mm on the 10th day of menstruation, but 72 hours after the first PRP infusion, the mean ET was 8.09 ± 2 mm. The mean differences of increasing ET was 1.28 ± 0.53 mm (P < 0.02). After 72 hour of infusion, endometrial vascularity was good in 9 patients (45%) and moderate in 11 patients (55%). Two weeks following IVF-ET, β-HCG test was positive in 9 patients (45%) and negative in 11 patients (55%).     

The use of autologous PRP significantly improved the ET and endometrial vascularity, and most importantly increased pregnancy rate and live birth rates in RIF patients.