Investigation of the Inhibitory Effect of Zinc Nanoparticles on Pseudomonas aeruginosa

Nowadays, the use of metals such as silver, copper, gold, and aluminum in various forms including nanoparticles as antimicrobial and bactericidal compounds has become popular. The present study aimed to evaluate the effect of zinc nanoparticles on ‌‌‌‌Pseudomonas aeruginosa (ATCC 27853).

In the current experimental study, zinc nanoparticles prepared from Nano Pars Co. ‌‌‌‌Pseudomonas aeruginosa ATCC 27853 was used as the indicator microorganism. The antibacterial activity of nano-particles was compared with benzalkonium using agar diffusion (disk diffusion) and dilution methods. Data were statistically analyzed using SPSS software, version 22 (IBM, Armonk, NY, USA) running one way ANOVA and Tukey test.

The results showed that the addition of zinc nanoparticles to the benzalkonium chloride solvent increased the diameter of the inhibitory zone of Pseudomonas spp. from 9±1 to 19.66 ± 3.05 )P-value =0.001). The MIC and MBC for zinc nanoparticles were 0.15 and 0.24 mg/ml, respectively, while the MIC and MBC for nanoparticles of zinc+benzalconium chloride 10% were 0.03 and 0.04, respectively. This indicates the excellent antibacterial activity of nanoparticles in combination with benzalkonium chloride solvent against Pseudomonas spp.

It seems that the stock of nanoparticles with benzalkonium chloride solvent has bactericidal or growth inhibitory effect against ‌‌‌‌Pseudomonas aeruginosa in different concentrations and has rapid bactericidal effect in concentrations higher than MIC concentration. Zinc oxide nanoparticles can be used to prevent or help treat ‌‌‌‌Pseudomonas aeruginosa infections.