Use of multioxidant solution for optimizing the disinfection of ornamental explants in the plant tissue culture laboratory of Mazare Novin Iranian Company

Contaminating microorganisms in tissue culture laboratories are from plant, human or environmental sources. The occurrence of this contamination of environmental origin occurs globally. The multioxidant solution for water disinfection, compared to other methods, has several advantages such as high disinfectant power, less chlorine residue in the water, improved taste and smell, biofilm removal and biosafety. The presence of above-mentioned various disinfecting factors causes the high efficiency of this solution. This solution is more effective than bleach and can be used for a variety of applications. Effect of different concentrations of multioxidant solution (0, 100, 200 and 400 ppm for 10, 20, 40 and 80 min) to control fungal and bacterial contamination of explants of three species of Gerbera, Phalanopsis orchid and Hawortia as well as the rate of fungal and bacterial contamination in the air after the use of anolyte (four concentrations of 0, 100, 200 and 400 ppm of anolyte solution using fogger for 5, 10, 15 and 20 min to Fog application) was examined at four times of the year in the commercial plant tissue culture laboratory of New Iranian Farms Company. The use of 400 ppm of anolytic multioxidant solution for 80 min of immersion in tissue culture explants can control fungal contamination in the explants. But bacterial control may not be completely eliminated depending on the plant species used. Therefore, the use of 70% ethanol solution for 30 to 60 seconds is recommended for disinfection of plant explants along with the use of anolyte. Another advantage of using this multioxidant solution is that the tissues do not burn, unlike the use of sodium hypochlorite, and this is most likely due to the neutral pH of this substance.