The Effect of Eight Weeks of Dorema Aucheri Extract Consumption and Aerobic Exercise on the Expression of Genes Involved in Bone Tissue Metabolism in Hypothyroid Mice

 Bone metabolic disorders are complex abnormalities that result from multiple factors, including genetic and environmental factors such as nutrition and physical activity. This study aimed to investigate the effects of aerobic exercise and Dorema Aucheri (DA) extract on the expression of genes involved in bone tissue metabolism under hypothyroid conditions.

 Thirty female C57BL/6 mice, aged 6-7 weeks, were divided into five groups: 1. Hypothyroidism, 2. Hypothyroid with DA extract (0.4 mg/kg administered by gavage for eight weeks), 3. Hypothyroidism with aerobic exercise, 4. Hypothyroidism with DA extract and aerobic exercise, 5. Control. Hypothyroidism was induced by intraperitoneal injection of propylthiouracil (8 mg/kg) for one month. The genes involved in bone metabolic disorders affected by hypothyroidism were identified using bioinformatics databases and analysis. Aerobic exercise was performed at a moderate to high intensity (45 minutes, zero inclines, 25 meters per minute). Gene expression was assessed using the quantitative Real-Time PCR method.

 Based on the results of the bioinformatics analysis, the genes RUNX2, PGC1OSX, PPAR-γ, SIRT1, MAPK3, and OPG were identified and investigated. In the hypothyroidism group compared to the control group, the expression of OPG and RUNX2 increased, while the expression of OSX decreased. Also, the expression of PGC1α, SIRT1, MAPK3, and PPAR-γ decreased under hypothyroid conditions (p<0.001). Treatment with DA extract and aerobic exercise significantly increased the expression levels of SIRT1, MAPK3, PGC1α, and PPAR-γ (p<0.001).

 DA extract and aerobic exercise have the potential to modulate the genetic network of bone metabolism and exert a positive regulatory effect on it.