The Effects of Phytase and Protease Supplementation in Diets Containing Seasame Meal on Nutrient Digestability, Bone Properties, Immunity and Blood Parameters in Broiler Chicken

This study was conducted to investigate the effects of supplemented phytase and protease enzymes in diets containing 15% of sesame meal on digestibility, bone physicochemical properties, immunity and blood parameters in broiler chicken. An experiment was performed in a completely randomized design using five dietary treatments with six replicates in which 300 one-day-old Ross 308 commercial broiler chicks were reared for 6 weeks. Dietary treatments were 1) corn-soybean meal based diet (negative control) 2) a corn-soybean meal diet including 15% sesame meal (positive control), 3) positive control diet supplemented with phytase, 4) positive control diet supplemented with protease and 5) positive control diet supplemented with both phytase and protease enzymes. The results indicated that ileal digestibility for crude protein was increased in chicks treated with enzymes (p < 0.05). The ileal digestibility for calcium and phosphorus were higher in chicks fed both phytase and protease (p < 0.05). Experimental treatments had no significant effects on the viscosity of gastrointestinal ileal digesta (p > 0.05). Tibial bone breaking strength in chicks treated with both enzymes increased (p < 0.05), also treatments containing phytase (with or without protease) led to more bone calcium and phosphorus (p < 0.05). Experimental treatments had no significant effect on bursa and spleen weights (p < 0.05). Blood cholestrol concentration in chicks treated with both enzymes were decreased (p < 0.05). Phytase (with or without protease) in diet, decreased blood triglyceride concentration (p < 0.05). Lower total protein was in chicks fed protease (p < 0.05). Blood calcium concentration in chicks fed enzymes were higher (p < 0.05). Supplementing broiler chicken diets with phytase and protease containing 15% sesame meal, had beneficial effects on protein, calcium and phosphorus digestibility, bone resistance, and some blood parameters.
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