Anticancer effect of digitalis nervosa hydroalcoholic extract on cervical cancer cell line (Hela)

Today, with the high prevalence of cancer, the need for drugs with fewer side effects and better therapeutic effects has been noticed by researchers, so that a large percentage of anti-cancer compounds are prepared from natural sources. The purpose of this study is to investigate the anticancer effects of digitalis nervosa hydroalcoholic extract on uterine cancer cell line (Hela).
In this experimental study, the hydroalcoholic extract of the marigold plant was prepared. Then, Hela cancer cell line was treated with different concentrations of the extract (3.125, 6.25, 12.5, 25, 50 and 100 μg/ml) for 24 hours and the cytotoxicity of the extract was evaluated by MTT method. Finally, the expression level of Bax, Bcl2, P53 and CDH1 genes at 50% lethality concentration was investigated by Time-Real PCR method. Data were analyzed using SPSS software and one-way variance test.
According to the results of the MTT test, the hydroalcoholic extract of foxglove has a dose-dependent cytotoxic effect against the cell line, and the concentration of 50% lethality was determined to be 24.67 micrograms per milliliter. Also, at the concentration (24.67 μg/ml), the expression of apoptotic genes Bax, CDH1 and P53 increased and the expression of Bcl2 gene decreased compared to the reference gene.
Thimble flower plant extract has cytotoxicity and apoptosis induction effect on Hela cancer cells.