Proteome analysis: identification of accumulated plant proteins in the gut of adults Aelia acuminate

Proteomics is a new technique in plant-insect interaction studies. Identification of effective proteins in the insect- plant systems is main challenge in the evolutionary ecology. In this study, accumulated plant proteins in the gut of Aelia acuminate were visualized using two dimensional electrophoresis. Targets spots were selected to cut, digest and blast in the mascot software using the National Center of Biotechnology Information database, respectively. Results showed that accumulated proteins were serpin, alpha- amylase inhibitors as plant inhibitors; peroxidase, dehydroascorbate reductase and deoxymuogenic acid synthase as antioxidant proteins; beta-amylase and agglutinin lecithin 3 as stored proteins; calmodulin as regulation protein and hypothetical protein as unknown protein. Accumulation of plant inhibitor proteins in the gut of adults proved that gut proteases of insect can be overcome to these toxic proteins. Results suggest that proteomics is effective technique to identify changed proteins in the insect and plants tissues and it will be helpful to discuss their physiological roles in the biochemical process.