Effects of plant extracts of Camellia sinensis and Thymus vulgaris on ‎sporulation of Eimeria oocysts of broiler exereta under laboratory ‎conditions

Coccidiosis is a parasitic protozoal disease throughout the world caused by Eimeria species. This disease is also common in Iran. There are many anticoccidial drugs, however; new drugs release are essential due to drug resistance. Green tea (Camellia sinensis) is a plant with antioxidant against diseases and thyme (Thymus vulgaris) has also antibacterial, antioxidant and antifungal effects. The anticoccidial drugs prescription are accompany with drug resistance and their poultry meat residue. Thus, the present study was aimed to investigate the effect of C. sinensis and T. vulgaris extracts on sporulation of Eimeria species oocysts of broiler chicken exereta. For this purpose, fecal samples were collected from broiler chicken farms of West Azerbaijan province, Iran. Oocysts were collected using floatation technique. A total number of 5×104 oocysts per mL exposed with different concentrations (20, 40, 60, 80 mg/ml) of C. sinensis and T. vulgaris in different times. Results indicated. C. sinensis and T. vulgaris extracts anticoccidial effect on sporulation of Eimeria oocysts was dose dependent. C. sinensis and T. vulgaris extracts inhibit sporulation of Eimeria oocysts at concentration of 80 and 60 mg/mL after 72h and 48h, respectively. It was concluded that C. sinensis and T. vulgaris extracts can inhibit sporulation of Eimeria oocysts under laboratory condition.