Investigation of the inhibitory effect of Lactobacillus acidophilus engineered probiotic expressing Tachyplesin I horseshoe peptide on the growth of Pseudomonas aeruginosa

Probiotics are living micro-organisms whose sufficient consumption causes health-giving effects to appear in the host's body. The purpose of this research is to produce the effects of Lactobacillus acidophilus probiotic bacteria expressing horseshoe crab Tachyplesin I on the growth of Pseudomonas aeruginosa.

Plasmid pNZ8148-Tachyplesin I containing Tachyplesin I gene was introduced into E.coli by heat shock method. The recombinant vector was introduced into Lactobacillus acidophilus bacteria by electroporation and screening was done by chloramphenicol antibiotic and PCR. Then, the inhibitory effect of the engineered Lactobacillus acidophilus bacteria on the growth of Pseudomonas aeruginosa was investigated and compared with antibiotic discs.

Plasmid pNZ8148-Tachyplesin I entry into Lactobacillus acidophilus was confirmed by chloramphenicol and PCR. Then it was determined that the engineered bacteria had an inhibitory effect on the growth of the pathogenic bacterium Pseudomonas aeruginosa, so that the diameter of the halo of non-growth created on Pseudomonas aeruginosa under the influence of the engineered Lactobacillus was acceptable. After comparing the halo diameters of ampicillin and chloramphenicol antibiotic discs with engineered Lactobacillus and conducting statistical analysis, no significant difference was reported in the non-growth halo (pvalue<0.05), but the halo diameter of tetracycline with The aura obtained from the engineered lactobacillus had a statistically significant difference.

Considering that the presence of the Tachyplesin I gene in Lactobacillus acidophilus was confirmed, the lack of growth caused by it around Pseudomonas aeruginosa indicates that the expression and secretion of the Lactobacillus Tachyplesin I gene has an antibiotic-like effect on Pseudomonas aeruginosa.