Anti-bacterial activity of eight monofloral Iranian honey types

Previously, we reported that the monoclonal Iranian honeys from different floral sources exhibited a large range from low to high anti-HIV activity. The aim of the present study was to determine the antibacterial activities of eight monofloral Iranian honeys.

the antibacterial activities of Iranian honeys were measured using disc diffusion and micro-broth dilution methods. The samples were evaluated spectrophotometrically for their total flavonoid content. the best results of within the in sillico antibacterial activity of the honeys with the most effective activity in vitro was performed by PyRx software. The molecular docking between flavonoids and 6 target proteins (DNAgyrase subunit B, penicilin binding protein, D-alanin D-alanin synthase, dihydrofolate reductase, and dihydropteroate synthetase and isoleucyl-tRNA synthetase) has been investigated.

The results showed that monofloral honeys from Zataria multiflora and Chamaemelum nobile showed the highest antibacterial effect against Bacillus subtilis, Streptococcus pyogenes, Proteus vulgaris, Bacillus licheniformis, Proteus mirabilis, and Staphylococcus saprophyticus. Monofloral Iranian honeys from Astragalus gummifer, Petro selenum sativum, Zizyphus Mauritiana, Citrus sinensis, Nigella sativa and Citrus aurantium flowers showed weak antibacterial activity. However, all these samples didn’t have any effect on Escherchia coli, Serratia marcescens, Salmonella enterica and Staphylococcus aureus. the entire flavonoid contents from Z. multiflora and C. Nobile were significantly quite other mono-floral honey types. The results of the docking study showed that each studied compound had appropriate interaction to targets. Analysis of docking results showed that flavonoids had the most effective results on 3SRW (dihydrofolate reductase) and 2ZDQ (D-alanineD-alanine ligase).

In conclusion, there's a connection between the anti-bacterial activity of mono-floral honey types and total flavonoids level Iranian honey types with high concentration of flavonoids like apigenin, quercetin and kaemferol may be good candidates for preclinical evaluation of anti-bacterial therapies.