Molecular Identification of Virulence Genes Salmonella Enterica Isolated of Animal and Human Diarrheal

Salmonella enterica is the leading cause of food poisoning in some countries. Salmonella species are the most prevalent causes of foodborne illness in humans and animals. S. enterica virulence genes were identified using PCR on 400 animal and human samples with specified primers. 6.25% percent of samples were examined by bacteriology and 16S rRNA. 12 (6%) animal and 13 (6.5%) human samples had S.enterica. All of isolates had invasive gene invA, Salmonella enterotoxin gene stn, and plasmid-encoded fimbriae pefA. Based on the results, the invA and stn virulence genes are stable in S. enterica cause diarrhea, and could be used on their own as a gene marker to quickly find virulent strains of S. enterica, while the pefA gene was only found in isolates from a few known sources. Testing for virulence genes with PCR revealed that the invA and stn genes are crucial for the serovars of S. enterica to be virulent in the host, demonstrating how harmful it is to feed these zoonotic organisms to people. S. enterica isolates appeared to be easily discovered using PCR assays that included the invA and stn virulence genes. In PCR tests, using the invA, pef, and stn virulence genes appears to be a quick, accurate, and precise way to distinguish S. enterica isolates.